Active Recombinant Rat CD28 protein, His-tagged

• Cat.No.: CD28-1876R
• Product Overview: Recombinant Rat CD28 protein(Asn 20 - Leu 150), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 20-150 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with Trehalose as protectant.
• Bio-activity: Loaded Biotinylated Human B7-1, Fc,Avitag on SA Biosensor, can bind Rat CD28, His Tag with an affinity constant of 15 μM as determined in BLI assay (ForteBio Octet Red96e).Loaded Biotinylated Human B7-2, Fc,Avitag on SA Biosensor, can bind Rat CD28, His Tag with an affinity constant of 6.1 μM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 16.9 kDa. The protein migrates as 30-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Asn20-Leu150
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd28 Cd28 molecule [ Rattus norvegicus ]
• Official Symbol: CD28
• Synonyms: CD28; Cd28 molecule; T-cell-specific surface glycoprotein CD28; CD28 antigen; CD28RNA
• Gene ID: 25660
• mRNA Refseq: NM_013121
• Protein Refseq: NP_037253