Recombinant Mouse Cd274 protein, His-tagged, Biotinylated, Primary Amine Labeling

• Cat.No.: Cd274-6854M
• Product Overview: Primary Amine Labeling Biotinylated Recombinant Mouse Cd274 protein(NP_068693)(Phe19-Thr238), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Phe19-Thr238
• Tag: C-His
• Conjugation/Label: Biotinl; Primary Amine Labeling
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Biotinylated Mouse PD-L1, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse PD-1 , hFc Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 25.9 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd274 CD274 antigen [ Mus musculus ]
• Official Symbol: Cd274
• Synonyms: CD274; CD274 antigen; programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• Gene ID: 60533
• mRNA Refseq: NM_021893
• Protein Refseq: NP_068693