Recombinant Mouse Cd274 Protein, mFc-tagged, low endotoxin, Alexa Fluor 647 conjugated

• Cat.No.: Cd274-359MAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant Mouse Cd274 protein (Phe19-Thr238)(low endotoxin), fused to mouse IgG2a Fc tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Species: Mouse
• Source: HEK293
• Tag: mFc
• Protein Length: 220
• Description: Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 51.8 kDa. The protein migrates as 65-85 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
• Endotoxin: < 0.1 EU/ μg by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Cd274
• Official Symbol: Cd274
• Synonyms: B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• Gene ID: 60533
• mRNA Refseq: NM_021893
• Protein Refseq: NP_068693
• UniProt ID: Q9EP73