Recombinant Mouse CD27 protein, Mouse IgG2a Fc-tagged, low endotoxin

• Cat.No.: CD27-357M
• Product Overview: Recombinant Mouse CD27 protein (Thr21-Arg182)(low endotoxin), fused to Mouse IgG2a Fc tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27/TNFRSF7 is found in most T-lymphocytes. CD27/TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.
• Source: HEK293
• Species: Mouse
• Tag: mIgG2a
• Form: Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 45.1 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: 162
• Endotoxin: Less than 0.1 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD27
• Official Symbol: CD27
• Synonyms: CD27; CD27 antigen; CD antigen 27; CD27L receptor; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7; S152; Tp55; Tnfrsf7
• Gene ID: 21940
• mRNA Refseq: NM_001033126
• Protein Refseq: NP_001028298
• UniProt ID: P41272