Recombinant Mouse Cd200r1 protein(Met1-Pro238), His&hFc-tagged

• Cat.No.: Cd200r1-631M
• Product Overview: Recombinant Mouse CD200R1 (NP_067300.1)(Met 1-Pro 238) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His&hFc
• Protein length: 1-238 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD200 at 1 μg/ml (100 μl/well) can bind mouse CD200R1/Fc with a linear range of 1.5-200 ng/ml.
• Molecular Mass: The recombinant mouse CD200R1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 462 amino acids and has a predicted molecular mass of 51.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmCD200R1/Fc monomer is approximately 90-100 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd200r1 CD200 receptor 1 [ Mus musculus ]
• Official Symbol: Cd200r1
• Synonyms: CD200R1; CD200 receptor 1; cell surface glycoprotein CD200 receptor 1; Orexin receptor 2; CD200 cell surface glycoprotein receptor; cell surface glycoprotein OX2 receptor 1; antigen identified by monoclonal MRC OX-2 receptor; OX2R; Mox2r; CD200R
• Gene ID: 57781
• mRNA Refseq: NM_021325
• Protein Refseq: NP_067300