Recombinant Human CD200R1 protein, hFc-tagged

• Cat.No.: CD200R1-785H
• Product Overview: Recombinant Human CD200R1 protein(AAQ19772)(Ala27-Leu266), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-hFc
• Protein length: Ala27-Leu266
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD200, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD200 R1, hFc Tag with the EC50 21.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 53.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.