Recombinant Mouse Cd14 protein, His-tagged

• Cat.No.: Cd14-58M
• Product Overview: Recombinant Mouse Cd14, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: Cd14, also known as monocyte differentiation antigen CD14, is a component of the innate immune system. This protein was found expressed in macrophages, neutrophil granulocyte and dendritic cells. It exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol tail (mCD14), the other a soluble form (sCD14). The major function is serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns.
• Source: Insect cell
• Species: Mouse
• Tag: His
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 38.3kDa (357aa)40-57kDa (SDS-PAGE under reducing conditions)
• AA Sequence: SPAPPEPCEL DEESCSCNFS DPKPDWSSAF NCLGAADVEL YGGGRSLEYL LKRVDTEADL GQFTDIIKSL SLKRLTVRAA RIPSRILFGALRVLGISGLQ ELTLENLEVT GTAPPPLLEA TGPDLNILNL RNVSWATRDA WLAELQQWLK PGLKVLSIAQ AHSLNFSCEQ VRVFPALSTLDLSDNPELGE RGLISALCPL KFPTLQVLAL RNAGMETPSG VCSALAAARV QLQGLDLSHN SLRDAAGAPS CDWPSQLNSL NLSFTGLKQVPKGLPAKLSV LDLSYNRLDR NPSPDELPQV GNLSLKGNPF LDSESHSEKF NSGVVTAGAP SSQAVALSGT LALLLGDRLF VHHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >90% by SDS – PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Cd14 CD14 antigen [ Mus musculus ]
• Official Symbol: Cd14
• Synonyms: CD14; CD14 antigen; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
• Gene ID: 12475
• mRNA Refseq: NM_009841
• Protein Refseq: NP_033971
• Pathway: Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; LPS transferred from LBP carrier to CD14, organism-specific biosystem
• Function: lipopolysaccharide binding; lipoteichoic acid binding