Recombinant Mouse Castor1 Protein, Myc/DDK-tagged

• Cat.No.: Castor1-1970M
• Product Overview: Purified recombinant protein of mouse full-length cytosolic arginine sensor for mTORC1 subunit 1 (Castor1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Functions as an intracellular arginine sensor within the amino acid-sensing branch of the TORC1 signaling pathway. As a homodimer or a heterodimer with CASTOR2, binds and inhibits the GATOR subcomplex GATOR2 and thereby mTORC1. Binding of arginine to CASTOR1 allosterically disrupts the interaction of CASTOR1-containing dimers with GATOR2 which can in turn activate mTORC1 and the TORC1 signaling pathway.
• Molecular Mass: 36.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Castor1 cytosolic arginine sensor for mTORC1 subunit 1 [ Mus musculus (house mouse) ]
• Official Symbol: Castor1
• Synonyms: Castor1; cytosolic arginine sensor for mTORC1 subunit 1; Gat; Gatsl3; 2410008K03Rik; cytosolic arginine sensor for mTORC1 subunit 1; GATS protein-like 3; GATS-like protein 3
• Gene ID: 71962
• mRNA Refseq: NM_028022
• Protein Refseq: NP_082298
• UniProt ID: Q9CWQ8