Recombinant Mouse Carboxypeptidase E, His-tagged

• Cat.No.: CPE-2891M
• Product Overview: Recombinant Mouse Carboxypeptidase E(NP_038522)(Met1-Phe476), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-476 a.a.
• Description: CarboxypeptidaseE (CPE), also known as Carboxypeptidase H or enkephalin convertase.Carboxypeptidase E cleaves C-terminal amino acid residues and is involved inneuropeptide processing. It is a peripheral membrane protein. CPEspecifically binds regulated secretory pathway proteins, includingprohormones, but not constitutively secreted proteins. Carboxypeptidase Eappears to have several functions. The active form of carboxypeptidase E wasshown to be in secretory vesicles, where it acts as an exopeptidase toactivate neuropeptides, It does that by cleaving off basic C-terminal aminoacids, producing the active form of the peptide. Products of carboxypeptidaseE include insulin, enkephalin, and most other neuroendocrine peptides.It hasalso been proposed that membrane-associated carboxypeptidase E acts as asorting signal for regulated secretory proteins in the trans-Golgi network ofthe pituitary and in secretory granules; regulated secretory proteins aremostly hormones and neuropeptides. Mice with mutant carboxypeptidase E Cpe,display endocrine disorders like obesity and infertility.
• Tag: C-His
• Form: Bulk protein in a0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulationor lyophilized powder. Normally 5-8% trehalose and mannitol are added asprotectants before lyophilization.
• Purity: >95% asdetermined by SDS-PAGE. All lots are greater than 95% pure.
• Storage: Store at -20°C in lyophilizedstate after receipt. For long term storage, upon reconstitution rmCPE shouldbe aliquot and store at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cpe carboxypeptidase E [ Mus musculus ]
• Official Symbol: CPE
• Synonyms: CPE; carboxypeptidase E; carboxypeptidase H; enkephalin convertase; prohormone-processing carboxypeptidase; CPH; fat; Cph1; Cph-1; R74677; MGC7101
• Gene ID: 12876
• mRNA Refseq: NM_013494
• Protein Refseq: NP_038522

Reviews

09/12/2024

CPE-2891M

The CPE is working very well! I will let you know when we plan to reorder next.

08/06/2024

CPE-2891M

Your product is working very well for us! Thank you for reaching out! We will let you know when we are ready to order next.