Recombinant Mouse Car12 protein, His-tagged

• Cat.No.: Car12-7847M
• Product Overview: Recombinant Mouse Car12 aa. (Ala161~Ile318 (Accession # Q8CI85)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Ala161~Ile318
• Description: This gene encodes a membrane-bound member of the alpha carbonic anhydrase family of enzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. These proteins participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Alternative splicing results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.6.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Car12 carbonic anhydrase 12 [ Mus musculus (house mouse) ]
• Official Symbol: Car12
• Synonyms: Car12; carbonic anhydrase 12; Ca12; CA-XII; AI314958; 2310047E01Rik; CA XII; carbonate dehydratase XII; carbonic anhydrase XII
• Gene ID: 76459
• mRNA Refseq: NM_001306148.1
• Protein Refseq: NP_001293077.1
• UniProt ID: Q8CI85