Recombinant Mouse Btc protein(Met1-Gln118), His&hFc-tagged

• Cat.No.: Btc-425M
• Product Overview: Recombinant Mouse BTC (NP_031594.1)(Met 1-Gln 118) was expressed in HEK293, fused with a C-terminal Fc region of Human IgG1 tag followed by a polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His&hFc
• Protein length: 1-118 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-10 ng/mL.
• Molecular Mass: The recombinant mouse BTC/Fc is a disulfide-linked homodimer. The reduced monomer consists of 335 amino acids and has a predicted molecular mass of 38 kDa. As a result of glycosylation, the apparent molecular mass of rm BTC/Fc monomer migrates with an apparent molecular mass of 50-55 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Btc betacellulin, epidermal growth factor family member [ Mus musculus ]
• Official Symbol: Btc
• Synonyms: BTC; betacellulin, epidermal growth factor family member; betacellulin; probetacellulin; Bcn
• Gene ID: 12223
• mRNA Refseq: NM_007568
• Protein Refseq: NP_031594