Recombinant Mouse Bmp2 protein, His-tagged

• Cat.No.: Bmp2-562M
• Product Overview: Recombinant Mouse Bmp2 (P21274) aa. (Gln281~Arg394) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Gln281~Arg394
• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. While a homozygous knockout mouse for this gene exhibits embryonic lethality, conditional knockout mice have defects in bone, cartilage and heart development.
• Form: Freeze-dried powder
• Molecular Mass: 15kDa as determined by SDS-PAGE reducing conditions
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 97%
• Characteristic: The isoelectric point is 8.2.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Storage buffer: 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: Bmp2 bone morphogenetic protein 2 [ Mus musculus (house mouse) ]
• Official Symbol: Bmp2
• Synonyms: Bmp2; bone morphogenetic protein 2; Bmp2a; AI467020; bone morphogenetic protein 2A
• Gene ID: 12156
• mRNA Refseq: NM_007553.3
• Protein Refseq: NP_031579.2
• UniProt ID: P21274