Recombinant Mouse Bdnf protein, His & GST-tagged

• Cat.No.: Bdnf-550M
• Product Overview: Recombinant Mouse Bdnf (P21237) aa. (Glu139~Arg249) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: GST&His
• Protein Length: Glu139~Arg249
• Description: The protein encoded by this gene is a member of the nerve growth factor family. It is involved in the growth, differentiation and survival of specific types of developing neurons both in the central nervous system (CNS) and the peripheral nervous system. It is also involved in regulating synaptic plasticity in the CNS. Expression of a similar gene in human is reduced in both Alzheimer's and Huntington disease patients. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.
• Form: Freeze-dried powder
• Molecular Mass: 43kDa as determined by SDS-PAGE reducing conditions
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 92%
• Characteristic: The isoelectric point is 8.5.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Bdnf brain derived neurotrophic factor [ Mus musculus (house mouse) ]
• Official Symbol: Bdnf
• Synonyms: Bdnf; brain derived neurotrophic factor; brain-derived neurotrophic factor
• Gene ID: 12064
• mRNA Refseq: NM_001048139.1
• Protein Refseq: NP_001041604.1
• UniProt ID: P21237