Recombinant Mouse Bcdin3d Protein, Myc/DDK-tagged
Cat.No. : | Bcdin3d-1846M |
Product Overview : | Purified recombinant protein of mouse full-length BCDIN3 domain containing (Bcdin3d), with C-terminal MYC/DDK tag, expressed in HEK293T cells. |
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Species : | Mouse |
Source : | HEK293 |
Tag : | DDK&Myc |
Description : | O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His)), acting as a capping enzyme by protecting tRNA(His) from cleavage by DICER1. Also able, with less efficiently, to methylate the 5' monophosphate of a subset of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Was also reported to mediate dimethylation of pre-miR-145; however dimethylation cannot be reproduced by another group which observes a monomethylation of pre-miR-145. |
Molecular Mass : | 32 kDa |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade after receiving vials. |
Concentration : | >50 μg/mL as determined by microplate BCA method |
Storage Buffer : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Gene Name | Bcdin3d BCDIN3 domain containing [ Mus musculus (house mouse) ] |
Official Symbol | Bcdin3d |
Synonyms | Bcdin3d; BCDIN3 domain containing; AV138748; 4930556P03Rik; RNA 5'-monophosphate methyltransferase; BCDIN3 domain-containing protein; pre-miRNA 5'-monophosphate methyltransferase; probable methyltransferase BCDIN3D; EC 2.1.1.- |
Gene ID | 75284 |
mRNA Refseq | NM_029236 |
Protein Refseq | NP_083512 |
UniProt ID | Q91YP1 |
◆ Recombinant Proteins | ||
BCDIN3D-611R | Recombinant Rat BCDIN3D Protein, His (Fc)-Avi-tagged | +Inquiry |
BCDIN3D-133H | Recombinant Human BCDIN3D Protein, GST-tagged | +Inquiry |
BCDIN3D-953R | Recombinant Rat BCDIN3D Protein | +Inquiry |
BCDIN3D-2329M | Recombinant Mouse BCDIN3D Protein | +Inquiry |
Bcdin3d-1846M | Recombinant Mouse Bcdin3d Protein, Myc/DDK-tagged | +Inquiry |
◆ Cell & Tissue Lysates | ||
BCDIN3D-8494HCL | Recombinant Human BCDIN3D 293 Cell Lysate | +Inquiry |
Not For Human Consumption!
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