Recombinant Mouse Atp5h Protein, Myc/DDK-tagged

• Cat.No.: Atp5h-1770M
• Product Overview: Purified recombinant protein of mouse full-length ATP synthase, H+ transporting, mitochondrial F0 complex, subunit D (Atp5h), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 18.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Atp5h ATP synthase, H+ transporting, mitochondrial F0 complex, subunit D [ Mus musculus (house mouse) ]
• Official Symbol: Atp5h
• Synonyms: ATP5H; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d; ATP synthase subunit d, mitochondrial; ATPase subunit d; 0610009D10Rik
• Gene ID: 71679
• mRNA Refseq: NM_027862
• Protein Refseq: NP_082138
• UniProt ID: Q9DCX2