Recombinant Mouse Atg9a Protein, Myc/DDK-tagged

• Cat.No.: Atg9a-1763M
• Product Overview: Purified recombinant protein of mouse full-length autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H. Required for carbonyl cyanide m-chlorophenylhydrazone (CCCP)-induced ATG8 family proteins lipidation, a key autophagy step.
• Molecular Mass: 63.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Atg9a autophagy related 9A [ Mus musculus (house mouse) ]
• Official Symbol: Atg9a
• Synonyms: Atg9a; autophagy related 9A; Apg; Atg9; Apg9l1; Atg9l1; AU019532; autophagy-related protein 9A; APG9 autophagy 9-like 1; APG9-like 1; autophagy protein 9; autophagy-related 9-like 1
• Gene ID: 245860
• mRNA Refseq: NM_001003917
• Protein Refseq: NP_001003917
• UniProt ID: Q68FE2