Recombinant Mouse ASK1, His-tagged, T7 tagged

• Cat.No.: ASK1-3585M
• Product Overview: Apoptosis Signal Regulating Kinase 1 (ASK1)

Specification

• Description: About the Marker: Effective Size Range: 10kDa to 70kDa.Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.Ready-to-use: No need to heat, dilute or add reducing agents before use.
• Source: E. Coli
• Species: Mouse
• Tag: His&T7
• Form: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Molecular Mass: 30.8kDa
• Protein length: 1380
• Endotoxin: <1.0eu per 1ug (determined by the lal</1.0eu
• Purity: >95%
• Applications: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Notes: Small volumes of ASK1 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: Map3k5 mitogen-activated protein kinase kinase kinase 5 [ Mus musculus ]
• Official Symbol: Map3k5
• Synonyms: ASK; ASK1; Mekk5; MAPKKK5; 7420452D20Rik; mitogen-activated protein kinase kinase kinase 5; ASK-1; MEKK 5; MEK kinase 5; MAPK/ERK kinase kinase 5; apoptosis signal-regulating kinase 1; mitogen activated protein kinase kinase kinase 5
• Gene ID: 26408
• mRNA Refseq: NM_008580
• Protein Refseq: NP_032606
• UniProt ID: O35099
• Chromosome Location: 10 A3; 10
• Pathway: Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Cellular Senescence, organism-specific biosystem
• Function: ATP binding; cysteine-type endopeptidase activator activity involved in apoptotic process