Recombinant Mouse Arachidonate 12-lipoxygenase, His-tagged

• Cat.No.: Alox12-584M
• Product Overview: Recombinant Mouse Alox12 fused with N-terminal his-tagged protein, was expressed using thebaculovirussystem. MW = 76.4 kDa.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Description: 12-Lipoxygenase(12-LO) catalyzes the formation of 12-HpETE from arachidonic acid. There are two main types of 12-LO, platelet-type and leukocyte-type, which share only 60% identity at the amino acid level and strongly differ in substrate specificity and enzyme kinetics. Leukocyte-type 12-LO has the ability to also produce 15-HpETE, however, platelet-type predominately forms 12-HpETE. Epidermal-type 12(S)- and 12(R)-LO enzymes have also been characterized, which catalyze the synthesis of their respective stereospecific 12-HETE products.
• Purity: ≥85%.
• Formulation: 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol.
• Stability: ≥6 months at -80°C; avoid freeze/thaw cycles by aliquoting the protein and storing at -80°C.

Gene Information

• Gene Name: Alox12 arachidonate 12-lipoxygenase [ Mus musculus ]
• Synonyms: Alox12; arachidonate 12-lipoxygenase; P-12LO; Alox12p; 9930022G08Rik; arachidonate 12-lipoxygenase, 12S-type; 12S-LOX; 12S-lipoxygenase; OTTMUSP00000006289; platelet-type lipoxygenase 12; EC 1.13.11.31
• Gene ID: 11684
• mRNA Refseq: NM_007440
• Protein Refseq: NP_031466
• UniProt ID: P39655
• Chromosome Location: 11 B3; 11 40.0 cM
• Pathway: Arachidonic acid metabolism; Metabolic pathways
• Function: arachidonate 12-lipoxygenase activity; iron ion binding; lipoxygenase activity; metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen