Recombinant Mouse Apob Protein, His-SUMO-tagged

• Cat.No.: Apob-494M
• Product Overview: Recombinant Mouse Apob Protein()(Leu32~Met306), fused with two N-terminal Tags, His-tag and SUMO-tag, was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His&SUMO
• Protein Length: Leu32~Met306
• Description: This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination.
• Form: 100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 50kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein.
• Notes: The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
• Storage: Avoid repeated freeze/thaw cycles.
  Store at 2-8ºC for one month.
  Aliquot and store at -80ºC for 12 months.
• Concentration: 200µg/mL
• Reconstitution: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Apob apolipoprotein B [ Mus musculus (house mouse) ]
• Official Symbol: Apob
• Synonyms: apob-; apob-48; AI315052; apob-100; Apo B-100
• Gene ID: 238055
• mRNA Refseq: NM_009693.2
• Protein Refseq: NP_033823.2
• UniProt ID: E9Q414