Recombinant Mouse Anpep protein(Lys66-Ser966), His-tagged

• Cat.No.: Anpep-905M
• Product Overview: Recombinant Mouse ANPEP (NP_032512.2)(Lys 66-Ser 966) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 66-966 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >800 pmoles/min/μg .
• Molecular Mass: The recombinant mouse ANPEP consists of 909 amino acids and has a calculated molecular mass of 104 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 120-130 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Anpep alanyl (membrane) aminopeptidase [ Mus musculus ]
• Official Symbol: Anpep
• Synonyms: ANPEP; alanyl (membrane) aminopeptidase; aminopeptidase N; aminopeptidase M; alanyl aminopeptidase; aminopeptidase N/CD13; membrane protein p161; microsomal aminopeptidase; Apn; AP-M; AP-N; Cd13; P150
• Gene ID: 16790
• mRNA Refseq: NM_008486
• Protein Refseq: NP_032512