Recombinant Mouse ANGPT2 protein(Lys275-Phe496), His-tagged

• Cat.No.: Angpt2-475M
• Product Overview: Recombinant Mouse ANGPT2 (NP_031452.2) (Lys275-Phe496) was expressed in HEK293 with a N-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Protein length: Lys275-Phe496
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Angiopoietin-2-His at 2 μg/ml (100 μl/well) can bind mouse TEK-Fc, the EC50 of mouse TEK-Fc is 350-900 ng/mL.
• Molecular Mass: The recombinant mouse ANGPT2 comprises 241 amino acids and has a predicted molecular mass of 27.5 kDa. The apparent molecular mass of the protein is approximately 33.4 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Angpt2 angiopoietin 2 [ Mus musculus ]
• Official Symbol: ANGPT2
• Synonyms: ANGPT2; angiopoietin 2; angiopoietin-2; Ang2; Agpt2; Ang-2
• Gene ID: 11601
• mRNA Refseq: NM_007426
• Protein Refseq: NP_031452