Recombinant Human ANGPT2 Protein, His-tagged

• Cat.No.: ANGPT2-696H
• Product Overview: Recombinant Human ANGPT2 Protein is produced by CHO cell expression system. This protein is fused with a His tag.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 19-496 aa
• Description: Angiopoietin-2, also known as ANGPT2, is a member of the ANG family. It has been identified as ligands for vascular endothelial-specific Tie2 receptor tyrosine kinase that interact with ANG2. This protein may be important growth factors in the generation of new blood vessels. Also, it is over-expression disrupts blood vessel formation in the mouse embryo. This protein is strongly expressed only at sites of vascular remodeling. It has been suggested that it may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression. Recombinant human Angiopoietin-2 fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10 % glycerol.
• Molecular Mass: 55.7 kDa
• AA Sequence: YNNFRKSMDS IGKKQYQVQH GSCSYTFLLP EMDNCRSSSS PYVSNAVQRD APLEYDDSVQ RLQVLENIME NNTQWLMKLE NYIQDNMKKEMVEIQQNAVQ NQTAVMIEIG TNLLNQTAEQ TRKLTDVEAQ VLNQTTRLEL QLLEHSLSTN KLEKQILDQT SEINKLQDKN SFLEKKVLAMEDKHIIQLQS IKEEKDQLQV LVSKQNSIIE ELEKKIVTAT VNNSVLQKQQ HDLMETVNNL LTMMSTSNSA KDPTVAKEEQ ISFRDCAEVFKSGHTTNGIY TLTFPNSTEE IKAYCDMEAG GGGWTIIQRR EDGSVDFQRT WKEYKVGFGN PSGEYWLGNE FVSQLTNQQR YVLKIHLKDWEGNEAYSLYE HFYLSSEELN YRIHLKGLTG TAGKISSISQ PGNDFSTKDG DNDKCICKCS QMLTGGWWFD ACGPSNLNGM YYPQRQNTNKFNGIKWYYWK GSGYSLKATT MMIRPADFHH HHHH
• Endotoxin: < 1.0 EU per 1 ug of protein (determined by LAL method)
• Purity: > 90 % by SDS - PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Absorbance at 280 nm)

Gene Information

• Gene Name: ANGPT2 angiopoietin 2 [ Homo sapiens ]
• Official Symbol: ANGPT2
• Synonyms: Angiopoietin-2 isoform a; ANGPT2; AGPT2; ANG2; angiopoietin 2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a
• Gene ID: 285
• mRNA Refseq: NM_001118887
• Protein Refseq: NP_001112359
• MIM: 601922
• UniProt ID: O15123