Recombinant Mouse Ang protein, His-tagged

• Cat.No.: Ang-519M
• Product Overview: Recombinant Mouse Ang (P21570) aa. (Gln25 ~Leu145) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the pancreatic ribonuclease A superfamily and is a potent inducer of neovascularization. The encoded protein is a secreted multifunctional tRNA-specific ribonuclease that promotes angiogenesis in response to angiogenetic stimuli such as hypoxia, mediates stress-induced translational repression by cleaving cellular tRNAs, stimulates cell proliferation by mediating rRNA transcription in prostate cancer cells, and is involved in neurite pathfinding. This gene resides in a cluster of highly related genes. It shares dual promoters and 5' exons with the ribonuclease, RNase A family 4 gene. Two alternatively spliced variants, with different 5' exons but the same coding exon, have been identified. Multiple pseudogenes have been found for this gene.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 15kDa as determined by SDS-PAGE reducing conditions
• Protein length: Gln25 ~Leu145
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 92%
• Characteristic: The isoelectric point is 9.4.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: Ang angiogenin, ribonuclease, RNase A family, 5 [ Mus musculus (house mouse) ]
• Official Symbol: Ang
• Synonyms: Ang; angiogenin, ribonuclease, RNase A family, 5; Ang1; Rnase5; Rnase5a; AI385586; angiogenin, ribonuclease A family, member 1; RNase 5; angiogenin-1; ribonuclease 5
• Gene ID: 11727
• mRNA Refseq: NM_001161731.2
• Protein Refseq: NP_001155203.1
• UniProt ID: P21570