Recombinant Mouse AGO2 protein, His-tagged

• Cat.No.: AGO2-2241M
• Product Overview: Recombinant Mouse AGO2 protein(Q8CJG0)(Met1-Ala860), fused with His tag, was expressed in Insect Cells.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Ala860
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse AGO2 consists of 878 amino acids and has a calculated molecular mass of 99 kDa. It migrates as an approximately 105 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.