Recombinant Mouse Agl Protein, His-tagged

• Cat.No.: Agl-93M
• Product Overview: Recombinant Mouse Agl Protein (Asp1197-Leu1532) with N-His tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Asp1197-Leu1532
• Description: Enables glycogen debranching enzyme activity; polysaccharide binding activity; and polyubiquitin modification-dependent protein binding activity. Acts upstream of or within glycogen metabolic process. Located in cytoplasm; inclusion body; and nucleus. Is expressed in several structures, including alimentary system; central nervous system; heart; musculature; and sensory organ. Used to study glycogen storage disease III. Human ortholog(s) of this gene implicated in glycogen storage disease III. Orthologous to human AGL (amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase).
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 42.4 kDa
  Accurate Molecular Mass: 44 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Agl amylo-1,6-glucosidase, 4-alpha-glucanotransferase [ Mus musculus (house mouse) ]
• Official Symbol: Agl
• Synonyms: AGL; amylo-1,6-glucosidase, 4-alpha-glucanotransferase; glycogen debranching enzyme; C77197; AI850929; 1110061O17Rik; 9430004C13Rik; 9630046L06Rik
• Gene ID: 77559
• mRNA Refseq: NM_001081326
• Protein Refseq: NP_001074795
• UniProt ID: F8VPN4