Recombinant Mouse Adipoq (Trimer), Flag-tagged

• Cat.No.: Adipoq-118M
• Product Overview: Recombinant Mouse Adipoq (trimer) was produced inHEK293. The cystenine 39 was replaced with alanine (C39A)9. mAd-C39A can only form trimer, but not hexamer or HMW form.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Flag
• Description: Adiponectin, also known as apM1, Acrp30, GBP28 and adipoQ, is a circulating hormone predominantly produced from adipose tissue. Many pharmacological studies demonstrated that this protein possesses potent anti-diabetic, anti-atherogenic and anti-inflammatory functions. Supplement of adiponectin protein can decrease blood glucose, improve insulin sensitivity, alleviate fatty liver and prevent atherosclerosis. The protein is post-translationally modified by hydroxylation and glycosylation, and forms three different oligomeric complexes in the circulation. Many clinical studies demonstrated that plasma adiponectin is an useful biomarker for metabolic syndrome, nonalcoholic steatohepatitis and certain type of cancers. Decreased circulating levels of plasma adiponectin (hypoadiponectinaemia) are associated with increased body mass index (BMI), decreased insulin sensitivity, less favourable plasma lipid profiles, increased levels of inflammatory markers and increased risk for the development of type 2 diabetes, hypertension, and coronary heart diseases.
• Amino Acid Sequence: EDDVTTTEEL APALVPPPKG TAAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYMYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN DYKDDDDK
• Form: Filtered (0.22 μm) and lyophilized in 1 mg/mL in PBS.
• Purity: >98%.
• Applications: Binding assay, ELISA, Ex vivo and in vivo activity analysis, In vitro, Western blotting.
• Quality Control Test: BCA to determine quantity of the protein. SDS PAGE to determine purity of the protein.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Gene Information

• Gene Name: Adipoq adiponectin, C1Q and collagen domain containing [ Mus musculus ]
• Synonyms: Adipoq; adiponectin, C1Q and collagen domain containing; APN; Acdc; apM1; 30kDa; GBP28; adipo; Acrp30; adiponectin
• Gene ID: 11450
• mRNA Refseq: NM_009605
• Protein Refseq: NP_033735
• UniProt ID: Q60994
• Chromosome Location: 16 16.0 cM
• Pathway: Adipocytokine signaling pathway; PPAR signaling pathway; Type II diabetes mellitus
• Function: hormone activity; protein binding; receptor binding