Recombinant Mouse Aco1 protein, His-tagged

• Cat.No.: Aco1-1702M
• Product Overview: Recombinant Mouse Aco1 aa. (Lys26~Val189 (Accession # P28271)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the aconitase/IPM isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Depending on iron levels in the cytosol, the encoded protein can function as either an aconitase enzyme or as an mRNA binding protein. When cellular iron levels are high, the encoded protein functions as an aconitase, an essential enzyme in the TCA cycle that catalyzes the conversion of citrate to isocitrate. When cellular iron levels are low, the encoded protein regulates iron uptake and utilization by binding to iron-responsive elements in the untranslated regions of mRNAs for genes involved in iron metabolism. Disruption of this gene is associated with pulmonary hypertension and polycythemia.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 20.4kDa
• Protein length: Lys26~Val189
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 7.1

Gene Information

• Gene Name: Aco1 aconitase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Aco1
• Synonyms: Aco1; aconitase 1; Irp1; Aco-1; Irebp; cytoplasmic aconitate hydratase; IRE-BP 1; citrate hydro-lyase; cytoplasmic aconitase; iron regulatory protein 1; iron-responsive element-binding protein 1
• Gene ID: 11428
• mRNA Refseq: NM_007386.2
• Protein Refseq: NP_031412.2
• UniProt ID: P28271