Recombinant Monkeypox virus (strain Zaire-96-I-16) E8L protein, His-tagged

• Cat.No.: E8L-010M
• Product Overview: Recombinant Monkeypox virus (strain Zaire-96-I-16) E8L protein(Q8V4Y0-1)(Met 1 - Thr 275), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Monkeypox virus (strain Zaire-96-I-16)
• Tag: C-His
• Protein length: Met 1 - Thr 275
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Monkeypox virus (strain Zaire-96-I-16) E8L, His Tag at 5 μg/mL (100 μL/well) can bind Anti-Monkeypox-E8L-Antibody with a linear range of 0.1-3 ng/mL.
• Molecular Mass: The protein has a calculated MW of 33.7 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.