Recombinant Monkey HAVCR2 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: HAVCR2-213CAF555
• Product Overview: A DNA sequence encoding the Monkey TIM3 (EHH54703.1) (Met1-Arg201) (Alexa Fluor 555 conjugated) was expressed with the Fc region of human IgG1 at the C-terminus.

Specification

• Description: Hepatitis A virus cellular receptor 2 (HAVCR2), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. TIM-3 thus serves as the surface molecule that distinguishes between the two effector populations derived from naive CD4(+) T-helper cells. TIM-3 is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. Th1 cells produce cytokines (IFN-gamma, interleukin-2, TNF-alpha and lymphotoxin) that are commonly associated with cellmediated immune responses against intracellular pathogens, delayedtype hypersensitivity reactions, and induction of organ-specific autoimmune diseases. It has been identified that galectin-9 is the Tim-3 ligand, and the TIM-3-galectin-9 pathway may serve to inhibit Th1 cellsduring a normal immune response and may be crucial for the induction of peripheral tolerance. Dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.
• Source: HEK293
• Species: Monkey
• Tag: Fc
• Form: Lyophilized
• Molecular Mass: The recombinant cynomolgus TIM3 comprises 418 amino acids and has a calculated molecular mass of 46.4 kDa. The apparent molecular mass of the protein is approximately 60.5 and 35.8 kDa in SDS-PAGE.
• Protein length: Met1-Arg201， 418
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: (70.9+26.4) % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH7.4.

Gene Information

• Gene Name: HAVCR2hepatitis A virus cellular receptor 2 [Macaca fascicularis(crab-eating macaque) ]
• Official Symbol: HAVCR2
• Synonyms: HAVCR2; hepatitis A virus cellular receptor 2
• Gene ID: 102141722
• mRNA Refseq: XM_005558381
• Protein Refseq: XP_005558438