Recombinant Monkey Colony Stimulating Factor 2 (granulocyte-macrophage)

• Cat.No.: CSF2-382M
• Product Overview: Recombinant MonkeyCSF2 is a single non-glycosylated polypeptide chain containing 127 aminoacids.

Specification

• Species: Monkey
• Source: E.coli
• Tag: Non
• Description: CSF2 was initiallycharacterized as a growth factor that can support the in vitro colonyformation of granulocyte-macrophage progenitors. It is produced by a numberof different cell types (including activated T cells, B cells, macrophages,mast cells, endothelial cells and fibroblasts) in response to cytokine orimmune and inflammatory stimuli. Besides granulocyte-macrophage progenitors,CSF2 is also a growth factor for erythroid, megakaryocyte and eosinophilprogenitors. On mature hematopoietic cells, CSF2 is a survival factor for andactivates the effector functions of granulocytes, monocytes, macrophages, andeosinophils.
• Form: Lyophilized from a0.2 µm filtered concentrated solution in PBS, pH 7.4.
• Molecular Weight: 14.4 kDa
• Sequence: APARSPSPGTQPWEHVNAIQ EARRLLNLSR DTAAEMNKTV EVVSEMFDLQEPSCLQTRLE LYKQGLQGSL TKLKGPLTMMASHYKQHCPP TPETSCATQIITFQSFKENL KDFLLVIPFD CWEPVQE
• Purity: > 98% by SDS-PAGEand HPLC analyses
• Biological Activity: Fully biologicallyactive when compared to standard. The ED50 as calculated by the dose-dependant stimulation of the proliferation of human TF-1 cells is less than0.1 ng/ml, corresponding to a Specific Activity of 1.0×10⁷ IU/mg.
• Endotoxin Level: < 0.1 ng/µg ofprotein
• Reconstitution: Centrifuge vialprior to opening. Reconstitute in sterile distilled water or aqueous buffercontaining 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutionsshould be apportioned into working aliquots and stored at ≤-20°C. Further dilutionsshould be made in appropriate buffered solutions.
• Storage: The lyophilizedprotein is stable at 2-4°C, but should be kept desiccated at -20°C for longterm storage. After reconstitution, the protein is stable for 1 week at2-4°C. For long term storage, aliquot and freeze at -80°C. Avoid repeatedfreeze-thaw cycles.
• OfficialSymbol: CSF2

Gene Information

• Gene Name: CSF2 colony stimulating factor 2(granulocyte-macrophage) [ Macaca mulatta ]
• Synonyms: CSF2; colony stimulating factor 2 (granulocyte-macrophage);GM-CSF
• Gene ID: 574371
• mRNA Refseq: NM_001032949
• Protein Refseq: NP_001028121
• Pathway: Amoebiasis; CytokineSignaling in Immune system; Cytokine-cytokine receptor interaction; Fcepsilon RI signaling pathway; HTLV-I infection; Hematopoietic cell lineage;Interleukin-3, 5 and GM-CSF signaling; Jak-STAT signaling pathway; Naturalkiller cell mediated cytotoxicity; Rheumatoid arthritis; Salmonellainfection; T cell receptor signaling pathway