Recombinant Leptotrichia wadeii Cas13a Protein, His-tagged

• Cat.No.: Cas13a-037L
• Product Overview: Recombinant Leptotrichia wadei Cas13a (LwC2c2) (1-1152) was expressed in E. coli with a C-terminal His tag.

Specification

• Description: Cas13a is RNA-guided RNA endonuclease that belongs to class 2 type VI CRISPR-Cas system. Cas13a possess both RNA recognition and cleavage activities and cleaves RNA through conserved basic residues within its two HEPN domains. Interestingly, activated Cas13a induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner. This property of Cas13a enzyme has been leveraged for the development of sensitive and robust nucleic acid detection diagnostic tool for clinical samples. Diagnostics using Cas13a for detection of SARS-CoV-2 offers promising alternative to quantitative PCR. In addition, CRISPR-Cas13a system provides opportunity for the development of clinical research in precision oncology.
• Source: E. coli
• Species: Leptotrichia wadeii (strain F0279)
• Tag: His
• Molecular Mass: 130 kDa
• Protein length: 1-1152
• Purity: ~ 90%
• Applications: ELISA
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein stored in 50mM sodium phosphate, pH 8.0, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol.
• Shipping: Dry ice

Gene Information

• Official Symbol: Cas13a
• Synonyms: LwCas13a; LwaCas13a; LwC2c2; EndoRNase; CRISPR-associated endoribonuclease Cas13a
• Protein Refseq: WP_021746774
• UniProt ID: U2PSH1