Recombinant Leptotrichia buccalis LbuCas13a Protein

• Cat.No.: LbuCas13a-21L
• Product Overview: Recombinant tag-free Leptotrichia buccalis LbuCas13a was expressed in E. coli cells.

Specification

• Description: Cas13a is an RNA-guided RNA endonuclease that belongs to the class 2 type VI CRISPR-Cas system. Cas13a possesses both RNA recognition and cleavage activities and cleaves RNA through conserved basic residues within its two HEPN domains). Interestingly, activated Cas13a induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner. Engineered LbuCas13a shows stability and altered cleavage activity in vitro and possesses the capability of robust exon skipping in human cells.
• Source: E. coli
• Species: Leptotrichia buccalis
• Molecular Mass: 132 kDa
• Purity: >80%
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.5 μg/μL
• Storage Buffer: Recombinant protein stored in 50 mM phosphate, pH 7.5, 300mM NaCl, 10% glycerol, 1 mM DTT.
• Shipping: Dry ice

Gene Information

• Synonyms: LbuCas13a
• Protein Refseq: WP_015770004