Recombinant Influenza A H7N9 Hemagglutinin/HA1 protein, His-tagged

• Cat.No.: HA-04I
• Product Overview: Recombinant Influenza A virus (A/Anhui/1-BALF_RG45/2013(H7N9)) HA1 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Influenza A
• Source: Insect Cells
• Tag: His
• Protein Length: 19-339
• Description: Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
• Form: Liquid
• Molecular Mass: 36 kDa (330aa)
• AA Sequence: DKICLGHHAVSNGTKVNTLTERGVEVVNATETVERTNIPRICSKGKRTVDLGQCGLLGTITGPPQCDQFLEFSADLIIERREGSDVCYPGKFVNEEALRQILRESGGIDKEAMGFTYSGIRTNGATSACRRSGSSFYAEMKWLLSNTDNAAFPQMTKSYKNTRKSPALIVWGIHHSVSTAEQTKLYGSGNKLVTVGSSNYQQSFVPSPGARPQVNGLSGRIDFHWLMLNPNDTVTFSFNGAFIAPDRASFLRGKSMGIQSGVQVDANCEGDCYHSGGTIISNLPFQNIDSRAVGKCPRYVKQRSLLLATGMKNVPEIPKGR
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95 % by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL
• Storage Buffer: PBS (pH 7.4) containing 10% glycerol

Gene Information

• Official Symbol: HA
• Synonyms: Hemagglutinin; HA
• UniProt ID: A0A024E3P0