Recombinant Influenza A H5N1 (A/Vietnam/1194/2004) HA Protein (ECD), His-tagged

• Cat.No.: HA-215H
• Product Overview: Recombinant extracellular domain of Influenza A virus (A/VietNam/1194/2004 (H5N1)) (AAT73273.1) hemagglutinin (Met 1-Gln 531) (Native, HA1+HA2, cleaved), fused with a C-terminal polyhistidine tag, was expressed in HEK293 Cells.

Specification

• Species: H5N1
• Source: HEK293
• Tag: His
• Protein Length: 1-531 a.a.
• Predicted N Terminal: Asp 17
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, 10% Glycerol, pH 8.0, 5% trehalose, 5% mannitol and 0.01% Tween80.
• Molecular Mass: The secreted recombinant hemagglutinin of Influenza A virus ((A/VietNam/1194/2004 (H5N1)) comprises 526 amino acids with the predicted molecular mass of 60 kDa. As a result of glycosylation, it migrates as an approximately 65-70 kDa band in Non-reduced SDS-PAGE, and two bands (25, 55 kDa) in reduced SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Official Symbol: HA
• Synonyms: HA; Hemagglutinin
• Protein Refseq: AAT73273.1