Recombinant Influenza A H5N1 (A/Common magpie/Hong Kong/2256/2006) HA Protein, His-tagged

• Cat.No.: HA-216H
• Product Overview: Recombinant extracellular domain (Met 1-Glu 530) of the influenza hemagglutinin (A/Common magpie/Hong Kong/2256/2006 (H5N1)) (ABJ96777.1) (HA1+HA2, uncleaved,with the cleavage site mutated (RRRKR-TETR)), fused with a C-terminal polyhistidine tag, was expressed in HEK293 Cells.

Specification

• Species: H5N1
• Source: HEK293
• Tag: His
• Protein Length: 1-530 a.a.
• Predicted N Terminal: Asp 17
• Form: Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.
• Molecular Mass: The secreted recombinant influenza A H5N1 (A/Common magpie/Hong Kong/2256/2006 (H5N1)) HA comprises 524 amino acids and has a predicted molecular mass of 59.6 kDa. As a result of glycosylation, it migrates as an approximately 70-80 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Official Symbol: HA
• Synonyms: HA; Hemagglutinin
• Protein Refseq: ABJ96777.1