Recombinant Influenza A H1N2 (A/swine/Iowa/A01049060/2010) HA protein(Met1-Ser523), His-tagged

• Cat.No.: HA-3351V
• Product Overview: Recombinant Influenza A virus (A/swine/Iowa/A01049060/2010(H1N2)) hemagglutinin (AEC23333) (Met1-Ser523), termed as HA, was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: H1N2
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ser523
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant hemagglutinin of the Influenza A virus (A/swine/Iowa/A01049060/2010(H1N2)) consists of 518 amino acids and predicts a molecular mass of 58.6 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.