Recombinant Influenza A H1N1 (A/WSN/1933) HA protein, His-tagged

• Cat.No.: HA-1579I
• Product Overview: Recombinant Influenza A H1N1 (A/WSN/1933) HA protein, His-taggedRecombinant Influenza A H1N1 (A/WSN/1933) HA protein(ACF54598.1)(Met1-Arg343), fused with His tag, was expressed in HEK293.

Specification

• Species: Influenza A H1N1 (A/WSN/1933)
• Source: HEK293
• Tag: His
• Protein Length: Met1-Arg343
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant HA1 subunit of influenza A H1N1 HA (A/WSN/1933 (H1N1)) comprises 337 amino acids and has a predicted molecular mass of 38 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 45-50 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.