Recombinant Influenza A H1N1 (A/California/04/2009) NA Protein, His-tagged

• Cat.No.: NA-224H
• Product Overview: Recombinant Influenza A virus (A/California/04/2009 (H1N1)) neuraminidase (ACP41107.1) (His36-Lys469), fused with a vasodilator-stimulated phosphoprotein tetramerization domain at the N-terminus and a polyhistidine tag at the C-terminus, was expressed in Baculovirus-Insect Cells.

Specification

• Species: H1N1
• Source: Insect Cells
• Tag: His
• Protein Length: 36-469 a.a.
• Predicted N Terminal: Asp
• Form: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, 10 % glycerol, pH 7.5, 5% trehalose, 5% mannitol and 0.01% Tween80.
• Molecular Mass: The recombinant Influenza A virus (A/California/04/2009 (H1N1)) NA consists of 480 amino acids and predicts a molecular mass of 53.6 kDa. As a result of glycosylation, it migrates as an approximately 58.7 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Official Symbol: NA
• Synonyms: NA; Neuraminidase
• Protein Refseq: ACP41107.1