Name: Recombinant Human ZNF454 293 Cell Lysate
SKU: ZNF454-69HCL

Species :	Human
Source :	HEK293
Tag :	Non
Description :	Antigen standard for zinc finger protein 454 (ZNF454) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Name	ZNF454 zinc finger protein 454 [ Homo sapiens ]
Official Symbol	ZNF454
Synonyms	ZNF454; zinc finger protein 454; FLJ37444; MGC138481;
Gene ID	285676
mRNA Refseq	NM_001178089
Protein Refseq	NP_001171560
UniProt ID	Q8N9F8
Chromosome Location	5q35.3
Pathway	Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem;