Recombinant Human ZAK, GST-tagged, Active

• Cat.No.: ZAK-457H
• Product Overview: Recombinant full-length human ZAK was expressed bybaculovirusinSf9 insect cellsusing an N-terminal GST tag. MW = 82 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: ZAK is a member of the MAPKKK family of signal transduction molecules and mediates gamma radiation signaling leading to cell cycle arrest. The activity of ZAK plays a role in cell cycle checkpoint regulation as well as being involved in regulating actin organization. Expression of kinase-dead ZAK in mouse fibroblasts disrupts actin stress fibers and causes morphologic changes. ZAK can activate JNK through MKK4/MKK7 and ERK5/p38-gamma via MKK3/MKK6. Expression of ZAK increases the population of cells in the G2/M phase of the cell cycle, whereas dominant-negative ZAK attenuated the G2 arrest caused by gamma radiation.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: ZAK sterile alpha motif and leucine zipper containing kinase AZK [ Homo sapiens ]
• Synonyms: sterile alpha motif and leucine zipper containing kinase AZK; ZAK; AZK; MLT; MRK; MLK7; MLTK; mlklak; MLK-related kinase; mixed lineage kinase 7; cervical cancer suppressor gene 4 protein; mixed lineage kinase-related kinase MRK-beta; MLK-like mitogen-activated protein triple kinase; mitogen-activated protein kinase kinase kinase MLT; leucine zipper- and sterile alpha motif-containing kinase; mixed lineage kinase with a leucine zipper and a sterile alpha motif; EC 2.7.11.25; HCCS-4; Leucine zipper- and sterile alpha motif-containing kinase ; Mixed lineage kinase-related kinase ; Sterile alpha motif- and leucine zipper-containing kinase AZK
• Gene ID: 51776
• mRNA Refseq: NM_016653
• Protein Refseq: NP_057737
• MIM: 609479
• UniProt ID: Q9NYL2
• Chromosome Location: 2q24.2
• Pathway: MAPK signaling pathway; Tight junction
• Function: ATP binding; MAP kinase kinase kinase activity; identical protein binding; magnesium ion binding; nucleotide binding; protein serine/threonine kinase activity; protein tyrosine kinase activity; transferase activity