Recombinant Human YY1 transcription factor, His-tagged

• Cat.No.: YY1-632H
• Product Overview: YY1, 1-414aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-414 a.a.
• Description: YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. This protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. Recombinant human YY1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In PBS,20% glycerol, 1mM DTT
• Molecular Mass: 47.1kDa (437aa) confirmed by MALDI-TOF(Molecular size on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASGDTL YIATDGSEMP AEIVELHEIE VETIPVETIE TTVVGEEEEE DDDDEDGGGG DHGGGGGHGH AGHHHHHHHH HHHPPMIALQ PLVTDDPTQV HHHQEVILVQ TREEVVGGDD SDGLRAEDGF EDQILIPVPA PAGGDDDYIE QTLVTVAAAG KSGGGGSSSS GGGRVKKGGG KKSGKKSYLS GGAGAAGGGG ADPGNKKWEQ KQVQIKTLEG EFSVTMWSSD EKKDIDHETV VEEQIIGENS PPDYSEYMTG KKLPPGGIPG IDLSDPKQLA EFARMKPRKI KEDDAPRTIA CPHKGCTKMF RDNSAMRKHL HTHGPRVHVC AECGKAFVES SKLKRHQLVH TGEKPFQCTF EGCGKRFSLD FNLRTHVRIH TGDRPYVCPF DGCNKKFAQS TNLKSHILTH AKAKNNQ
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: YY1 YY1 transcription factor [ Homo sapiens ]
• Official Symbol: YY1
• Synonyms: YY1; YY1 transcription factor; transcriptional repressor protein YY1; DELTA; INO80 complex subunit S; INO80S; NF E1; UCRBP; Yin and Yang 1 protein; YIN YANG 1; YY-1; delta transcription factor; NF-E1; YIN-YANG-1
• Gene ID: 7528
• mRNA Refseq: NM_003403
• Protein Refseq: NP_003394
• MIM: 600013
• UniProt ID: P25490
• Chromosome Location: 14q
• Pathway: Delta-Notch Signaling Pathway, organism-specific biosystem; E2F transcription factor network, organism-specific biosystem; Notch signaling pathway, organism-specific biosystem; Notch-mediated HES/HEY network, organism-specific biosystem; Signaling events mediated by HDAC Class I, organism-specific biosystem; mTOR signaling pathway, organism-specific biosystem; p53 pathway, organism-specific biosystem
• Function: DNA binding; RNA binding; four-way junction DNA binding; metal ion binding; protein binding; sequence-specific DNA binding transcription factor activity; transcription coactivator activity; transcription corepressor activity; transcription regulatory regi