Recombinant Human YAP1 protein, GST-tagged

• Cat.No.: YAP1-33H
• Product Overview: Recombinant Human YAP1 protein(206-379 aa), fused with GST Tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: GST
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 100mM GSH.
• Protein length: 206-379 aa
• AA Sequence: MLSQMNVTAPTSPPVQQNMMNSASG PLPDGWEQAMTQDGEIYYINHKNKT TSWLDPRLDPRFAMNQRISQSAPVK QPPPLAPQSPQGGVMGGSNSNQQQQ MRLQQLQMEKERLRLKQQELLRQAM RNINPSTANSPKCQELALRSQLPTL EQDGGTQNPVSSPGMSQELRTMTT
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: YAP1 Yes-associated protein 1 [ Homo sapiens ]
• Official Symbol: YAP1
• Synonyms: YAP1; Yes-associated protein 1; Yes associated protein 1, 65kDa; yorkie homolog; YAP65; yes-associated protein 2; yes-associated protein beta; yes-associated protein delta; 65 kDa Yes-associated protein; YAP; YKI; YAP2
• Gene ID: 10413
• mRNA Refseq: NM_001130145
• Protein Refseq: NP_001123617
• MIM: 606608
• UniProt ID: P46937

Reviews

11/19/2022

This short half-life and high clearance of YAP1 allow it to penetrate the tissue and reach the lesion site, which improves the therapeutic effect.

09/23/2022

The green production process of YAP1 not only improves the production efficiency, but also minimizes the environmental impact, truly realizing a win-win situation of economic and environmental benefits.

10/13/2019

The potent biological activity of YAP1 combined with its high purity makes the experimental results highly reliable.

Q&As

What are the research and development directions of anti-tumor drugs for YAP1 protein?

The research and development directions of antitumor drugs of YAP1 protein include inhibitors targeting YAP1, drugs that regulate the downstream signaling pathway of YAP1, and combination drug strategies.

In which signaling pathways are YAP1 proteins regulated?

YAP1 protein is regulated in multiple signaling pathways such as Wnt/β-catenin, Hippo, and FGF, thereby affecting cell proliferation and growth.

What signaling pathways does the YAP1 protein play a key role?

YAP1 protein plays a key role in the HIPPO signaling pathway, and its activation or inactivation affects cell proliferation, apoptosis, and differentiation.

How does the YAP1 protein participate in tumor invasion and metastasis?

YAP1 protein participates in tumor invasion and metastasis by regulating epithelial-mesenchymal transformation, cell migration and the expression of invasion-related factors.

How is YAP1 protein involved in the regulation of cell proliferation and growth?

The YAP1 protein is involved in regulating cell proliferation and growth, mainly through nuclear translocation and interaction with nuclear transcription factors to regulate the expression of related genes.

What are the effects of post-translational modifications of the YAP1 protein?

Post-translational modifications of YAP1 protein, such as phosphorylation and acetylation, can regulate its transcriptional activity and subcellular localization, thereby affecting tumor development.