Recombinant Human Y box binding protein 1, His-tagged

• Cat.No.: YBX1-636H
• Product Overview: YBX1, 1-324aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-324 a.a.
• Description: YBX1, also known as Y box binding protein 1, associates with the selenocysteine insertion sequence and functions in mammalian selenoprotein translation. This protein is a potential drug target in cancer therapy. It helps the replication of adenovirus type 5, a commonly used vector in gene therapy. Thus, YBX1 can cause an "oncolytic" effect in YBX1 positive cancer cells treated with adenoviruses. Recombinant human YBX1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Form: Liquid. Phosphate Buffered Saline (pH7.4) containing 20% glycerol
• Molecular Mass: 38.3 kDa (347aa) confirmed by MALDI-TOF(Molecular size on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSEAET QQPPAAPPAA PALSAADTKP GTTGSGAGSG GPGGLTSAAP AGGDKKVIAT KVLGTVKWFN VRNGYGFINR NDTKEDVFVH QTAIKKNNPR KYLRSVGDGE TVEFDVVEGE KGAEAANVTG PGGVPVQGSK YAADRNHYRR YPRRRGPPRN YQQNYQNSES GEKNEGSESA PEGQAQQRRP YRRRRFPPYY MRRPYGRRPQ YSNPPVQGEV MEGADNQGAG EQGRPVRQNM YRGYRPRFRR GPPRQRQPRE DGNEEDKENQ GDETQGQQPP QRRYRRNFNY RRRRPENPKP QDGKETKAAD PPAENSSAPE AEQGGAE
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: YBX1 Y box binding protein 1 [ Homo sapiens ]
• Official Symbol: YBX1
• Synonyms: YBX1; Y box binding protein 1; NSEP1, nuclease sensitive element binding protein 1; nuclease-sensitive element-binding protein 1; BP 8; CSDA2; CSDB; DBPB; MDR NF1; NSEP 1; YB 1; YB1; CBF-A; EFI-A; DNA-binding protein B; Y-box-binding protein 1; Y-box transcription factor; enhancer factor I subunit A; nuclease sensitive element binding protein 1; CCAAT-binding transcription factor I subunit A; major histocompatibility complex, class II, Y box-binding protein I; BP-8; YB-1; NSEP1; NSEP-1; MDR-NF1; MGC104858; MGC110976; MGC117250
• Gene ID: 4904
• mRNA Refseq: NM_004559
• Protein Refseq: NP_004550
• MIM: 154030
• UniProt ID: P67809
• Chromosome Location: 1p34
• Pathway: Gene Expression, organism-specific biosystem; IL-2 Signaling Pathway, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; SIDS Susceptibility Pathways, organism-specific biosystem; Signaling events mediated by PTP1B, organism-specific biosystem; mRNA Splicing, organism-specific biosystem; mRNA Splicing - Major Pathway, organism-specific biosystem
• Function: DNA binding; DNA binding; RNA binding; RNA binding; double-stranded DNA binding; protein binding; sequence-specific DNA binding transcription factor activity; single-stranded DNA binding