YBX1
-
Official Full Name
Y box binding protein 1 -
Overview
Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.<br/>The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation. -
Synonyms
YBX1;Y box binding protein 1;YB1;BP-8;CSDB;DBPB;YB-1;CSDA2;NSEP1;NSEP-1;MDR-NF1;nuclease-sensitive element-binding protein 1;CBF-A;EFI-A;DNA-binding protein B;Y-box-binding protein 1;Y-box transcription factor;enhancer factor I subunit A;nuclease sensitive element binding protein 1;CCAAT-binding transcription factor I subunit A;major histocompatibility complex, class II, Y box-binding protein I
Recombinant Proteins
- Human
- Mouse
- Chicken
- Rat
- Zebrafish
- E.coli
- Mammalian Cells
- Wheat Germ
- HEK293
- Yeast
- In Vitro Cell Free System
- GST
- His
- DDK
- Myc
- Non
- Avi
- Fc
- Flag
Background
What is YBX1 Protein?
YBX1, short for Y-box binding protein 1, is a bit like a Swiss Army knife for our cells. It has a hand in all sorts of cellular activities, which makes it a pretty big deal. You'll spot it hanging out in both the nucleus and the cytoplasm, where it juggles tasks like switching genes on and off and keeping RNA stable. YBX1 steps up during stress and can even play a part in cancer by messing with how cells grow and divide. In some cancer cases, lots of YBX1 is tied to more aggressive tumors and worse outcomes, so researchers are keen on studying it for both basic cell function and cancer treatments.What is the Function of YBX1 Protein?
YBX1, or Y-box binding protein 1, plays a crucial role in our cells, acting like a multitasking tool. One of its main jobs is helping regulate gene expression, which means it helps decide when genes get switched on or off. This protein also stabilizes RNA messages, which are the instructions our cells use to make proteins. It hangs out in both the nucleus and the cytoplasm, making sure everything runs smoothly. During stress, YBX1 kicks in to help the cell deal with challenges. In cancer research, YBX1 has caught attention because it can affect how cancer cells grow and spread, with high levels often linked to more aggressive tumors. So, it's not just a fundamental part of cellular machinery but also a potential key to tackling some cancers.
Fig1. Role of YBX1 in pancreatic cancer. (Ngoc Thi Minh Dinh, 2024)
YBX1 Related Signaling Pathway
The YBX1 protein is a key player in several signaling pathways that help keep our cells ticking. It’s primarily known for its role in gene expression regulation. YBX1 hangs out in the nucleus and can bind to specific DNA sequences called Y-boxes, influencing which genes get turned on or off. This impacts everything from cell growth and division to how cells respond to stress. In cancer pathways, YBX1 is pretty influential because it affects how cancer cells proliferate and survive. Its involvement in these pathways often results in promoting tumor growth and resistance to therapy. Because of this, researchers are very interested in YBX1 as a potential target for cancer treatments, aiming to tweak its pathway to hinder aggressive tumor behavior and improve patient outcomes.YBX1 Related Diseases
YBX1 protein pops up in a number of diseases, mostly because it's deeply involved in how cells grow and respond to stress. In cancer, YBX1 is often a major player. High levels of this protein are usually linked to tumors getting more aggressive, which makes the cancer harder to treat. It seems to help cancer cells keep dividing unchecked, dodge death, and resist therapies. Besides cancer, YBX1 is also involved in other conditions, like when cells react to inflammation or stress in disorders. Because of its big role in such processes, scientists are looking into YBX1 not just to understand these diseases better but also as a possible target for new treatments that could interrupt its influence on disease progression.Bioapplications of YBX1
YBX1 has some exciting bioapplications, especially in the world of medical research and treatment development. Since this protein is heavily involved in regulating genes and stabilizing RNA, it's at the center of understanding various diseases, notably cancer. Researchers look at YBX1 to figure out how cancer cells manage to grow aggressively and resist treatments. This makes it a hot target for potentially developing new therapies that can either dial down its activity or block it altogether, aiming to slow tumor growth and improve patient outcomes. Beyond cancer, YBX1's role in cellular stress responses and inflammation means it could also be key in treating other conditions where these processes are out of whack. So, whether it's about crafting targeted cancer therapies or tackling inflammatory diseases, YBX1 has a lot to offer in advancing biomedical innovation.Case Study
Case Study 1: Lu K. et al. Liver Int. 2024
Metabolic dysfunction-associated steatotic liver disease (MASLD) leads in chronic liver issues, but its mechanisms remain vague. We found a lncRNA called LNCHC, studying its role in MASLD. LNCHC binds with YBX1, preventing its breakdown and stabilizing PNPLA3 mRNA, which cuts liver fat. Though LNCHC, YBX1, and PNPLA3 help control lipid buildup, they may also worsen metabolic dysfunction-related liver inflammation.-
Fig1. CO-IP assay was performed to detect the ubiquitination levels of YBX1 in the BRL cells and LO2 cells transfected with NC or LNCHC siRNA. -
Fig2. Western blotting analysis of YBX1 expression levels in YBX1-knockdown LO2 cells.
Case Study 2: Chiba M. et al. Int J Mol Sci. 2023
Aging cells release inflammatory proteins and small vesicles called SASP, which contribute to age-related diseases. They produce SATII RNA, ramping up inflammatory genes and moving in vesicles to other cells. We found YBX1 helps SATII RNA get into these vesicles. Cells receiving these vesicles age faster and express more inflammatory genes. Cutting YBX1 levels reduces SATII RNA in vesicles and limits SASP spread. Higher YBX1 is found in cancerous tissues and is linked to worse outcomes in breast and ovarian cancers.-
Fig3. Western blot analysis was performed after RNA pull-down by SATα RNA or SATII RNA using each fraction. -
Fig4. Western blot analysis of whole cell lysate of siCtrl and YBX1 knockdown RPE-1 cells.
Quality Guarantee
High Purity
-
.jpg)
Fig1. SDS-PAGE (YBX1-1557M) -
.jpg)
Fig2. SDS-PAGE (YBX1-4852H)
Involved Pathway
YBX1 involved in several pathways and played different roles in them. We selected most pathways YBX1 participated on our site, such as BDNF signaling pathway,Gene Expression,Processing of Capped Intron-Containing Pre-mRNA, which may be useful for your reference. Also, other proteins which involved in the same pathway with YBX1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Signaling events mediated by PTP1B | CSN2,NOX4,DOK1 |
| mRNA Splicing - Minor Pathway | ZMAT5,SF3B2,SNRNP35,TXNL4A,PRPF8,ZCRB1,SNRNP25,PDCD7,SF3B4,SNRNP48 |
| BDNF signaling pathway | FRS2,CSNK2A1,EEF2,EIF4EBP1,EIF4E,CFL1,GNB2L1,MAP3K2,DOK5,EGR2 |
| Processing of Capped Intron-Containing Pre-mRNA | SNRNP48,SF3A3,PTBP1,SRSF11,HNRNPL2,THOC4,CD2BP2,BCAS2,HNRNPA0B,SNRNP35 |
| mRNA Splicing | HNRPL,HNRNPH1,PCBP2,CD2BP2,HNRNPD,HNRNPA0,SUGP1,TXNL4A,HNRNPF,SF3B2 |
| Gene Expression | BARHL1,POU2F1B,TSNAX,POLR3G,TSEN15,ZFP36L1B,TH1L,NR2F6B,DPY19L2,ARHGEF38 |
| mRNA Splicing - Major Pathway | PTBP1B,HNRNPH2,SFRS3A,HNRNPA0,HNRNPL2,RBM5,HNRNPL,HNRNPA2B1,PTBP1A,TXNL4A |
| SIDS Susceptibility Pathways | CHRFAM7A,RUNX3,PKNOX1,CTCF,TAC1,ECE1,CREM,PHOX2A,CC2D1A,PLP1 |
-
Fig1. YBX1 as a neural input. (Ngoc Thi Minh Dinh, 2024) -
Fig2. YBX1 plays a crucial role in cell cycle regulation. (Ngoc Thi Minh Dinh, 2024)
Protein Function
YBX1 has several biochemical functions, for example, DNA binding,GTPase binding,RNA binding. Some of the functions are cooperated with other proteins, some of the functions could acted by YBX1 itself. We selected most functions YBX1 had, and list some proteins which have the same functions with YBX1. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| double-stranded DNA binding | MTERF2,XRCC3,RAD51D,RBMS1,ERCC5,HNRNPDL,FBXO18,RAD51L1,HES2,TP53 |
| chromatin binding | PCGF2,CREB3,ZMYND11,TOX4,MCM5,HDAC5,ATOH1A,CRX,NFATC2,MB21D2 |
| transcription factor activity, sequence-specific DNA binding | ENO1,TBX1,ZNF26,STAT1B,HSF5,NR1H4,ZBTB48,CSRNP1,KDM3A,BHLHE40 |
| single-stranded DNA binding | ALYREF,NABP2,PURA,RAD51,ERCC5,RAD51C,CTC1,HNRPDL,MCM6,OBFC1 |
| protein binding | PDZD3,DZIP1,IGF2BP3,SMARCE1,ZNF180,SNX1,RPLP2,COBRA1,GLMN,HP |
| transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding | DBP,TP63,ARID3B,HIF1A,TFAP4,HOXC11,STAT3,ELK1,E2F4,GMEB1 |
| GTPase binding | HSP90AA1,VPS41,LCP1,SPTBN1,SH3GL1,BNIP3,ENO1,GNB1,GOLGA4,STOML2 |
| RNA polymerase II core promoter proximal region sequence-specific DNA binding | CEBPZ,TP73,FEZF2,JUND,CREB3L2,ISL1,PHOX2B,RFX3,PAX5,SOX1 |
| RNA binding | UBR5,NOM1,LRPPRC,PSMA1,LIN28A,SRSF3,EXOSC3,SRSF1B,PRDM14,CSTF3 |
Interacting Protein
YBX1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with YBX1 here. Most of them are supplied by our site. Hope this information will be useful for your research of YBX1.
SYNCRIP;C1QBP;HNRNPR;DHX9
Resources
Related Services
Related Products
References
- Blackwell, E; Ceman, S; et al. A new regulatory function of the region proximal to the RGG box in the Fragile X mental retardation protein. JOURNAL OF CELL SCIENCE 124:3060-3065(2011).
