Recombinant Human XYLT2 protein, His & T7-tagged

• Cat.No.: XYLT2-7937H
• Product Overview: Recombinant Human XYLT2 aa. (Phe596~Arg865 (Accession # Q9H1B5)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Phe596~Arg865
• Description: The protein encoded by this gene is an isoform of xylosyltransferase, which belongs to a family of glycosyltransferases. This enzyme transfers xylose from UDP-xylose to specific serine residues of the core protein and initiates the biosynthesis of glycosaminoglycan chains in proteoglycans including chondroitin sulfate, heparan sulfate, heparin and dermatan sulfate. The enzyme activity, which is increased in scleroderma patients, is a diagnostic marker for the determination of sclerotic activity in systemic sclerosis. Alternatively spliced transcript variants have been found for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.9kDa.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.1.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: XYLT2 xylosyltransferase 2 [ Homo sapiens (human) ]
• Official Symbol: XYLT2
• Synonyms: XYLT2; xylosyltransferase 2; SOS; XT2; XT-II; PXYLT2; xylT-II; UDP-D-xylose:proteoglycan core protein beta-D-xylosyltransferase; peptide O-xylosyltransferase 1; protein xylosyltransferase 2; xylosyltransferase II
• Gene ID: 64132
• mRNA Refseq: NM_022167.3
• Protein Refseq: NP_071450.2
• UniProt ID: Q9H1B5