Recombinant Human XRCC6 293 Cell Lysate

• Cat.No.: XRCC6-253HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for X-ray repair complementing defective repair in Chinese hamster cells 6 (XRCC6) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: XRCC6 X-ray repair complementing defective repair in Chinese hamster cells 6 [ Homo sapiens ]
• Official Symbol: XRCC6
• Synonyms: XRCC6; X-ray repair complementing defective repair in Chinese hamster cells 6; G22P1, thyroid autoantigen 70kD (Ku antigen) , thyroid autoantigen 70kDa (Ku antigen); X-ray repair cross-complementing protein 6; D22S671; D22S731; Ku autoantigen; 70kDa; KU70; ML8; 5-dRP lyase Ku70; Ku autoantigen, 70kDa; DNA repair protein XRCC6; Ku autoantigen p70 subunit; 70 kDa subunit of Ku antigen; thyroid-lupus autoantigen p70; lupus Ku autoantigen protein p70; 5-deoxyribose-5-phosphate lyase Ku70; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; thyroid autoantigen 70kD (Ku antigen); ATP-dependent DNA helicase 2 subunit 1; thyroid autoantigen 70kDa (Ku antigen); ATP-dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase II, 70 kDa subunit; TLAA; CTC75; CTCBF; G22P1
• Gene ID: 2547
• mRNA Refseq: NM_001469
• Protein Refseq: NP_001460
• MIM: 152690
• UniProt ID: P12956
• Chromosome Location: 22q13.2
• Pathway: 2-LTR circle formation, organism-specific biosystem; BARD1 signaling events, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; DNA Repair, organism-specific biosystem; DNA-PK complex, organism-specific biosystem; Disease, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem