Recombinant Human XRCC5, His tagged

• Cat.No.: XRCC5-1798H
• Product Overview: Human XRCC5 (P13010) (Met 1-Ile 732), fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; Human XRCC6 (P12956) (Met 1-Asp 609), fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-732;1-609 a.a.
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0
• Molecular Mass: The recombinant heterodimer of human XRCC5/XRCC5 comprises 1379 (751 + 628) amino acids and has a calculated molecular mass of 157 (85 + 72) kDa. The apparent molecular mass of rh XRCC5/XRCC5 heterodimer is approximately 70 & 85 kDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: XRCC5 X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) [ Homo sapiens ]
• Official Symbol: XRCC5
• Gene ID: 7520
• mRNA Refseq: NM_021141
• Protein Refseq: NP_066964
• MIM: 194364
• UniProt ID: P13010