Recombinant Human WWP1, GST-tagged

• Cat.No.: WWP1-75H
• Product Overview: Recombinant full-length human WWP1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: WWP1 or WW domain containing E3 ubiquitin protein ligase 1 belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. WWP1 are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. WWP1 negatively regulates TGF-beta signaling by directing the degradation of active components of the TGF-beta network. Wwp1 and Ubc18 function to ubiquitinate substrates that regulate longevity induced by diet restriction.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~160 kDa
• Purity: >70% by densitometry.
• Applications: Western Blot
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.05 μg/μl

Gene Information

• Gene Name: WWP1 WW domain containing E3 ubiquitin protein ligase 1 [ Homo sapiens ]
• Official Symbol: WWP1
• Synonyms: WWP1; WW domain containing E3 ubiquitin protein ligase 1; NEDD4-like E3 ubiquitin-protein ligase WWP1; AIP5; DKFZP434D2111; WW domain-containing protein 1; atrophin-1 interacting protein 5; atrophin-1-interacting protein 5; TGIF-interacting ubiquitin ligase 1; Nedd-4-like ubiquitin-protein ligase; Tiul1; hSDRP1; DKFZp434D2111
• Gene ID: 11059
• mRNA Refseq: NM_007013
• Protein Refseq: NP_008944
• MIM: 602307
• UniProt ID: Q9H0M0
• Chromosome Location: 8q21
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Downregulation of ERBB4 signaling, organism-specific biosystem; Endocytosis, organism-specific biosystem
• Function: acid-amino acid ligase activity; ligase activity; protein binding; ubiquitin-protein ligase activity; ubiquitin-protein ligase activity