Recombinant Human WARS2 protein, His & T7-tagged

• Cat.No.: WARS2-1273H
• Product Overview: Recombinant Human WARS2 aa. (Met1~Leu360 (Accession # Q9UGM6) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• ProteinLength: Met1~Leu360
• Description: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. This gene encodes the mitochondrial tryptophanyl-tRNA synthetase. Two alternative transcripts encoding different isoforms have been described.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 43.8kDa.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 9.3.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: WARS2 tryptophanyl tRNA synthetase 2, mitochondrial [ Homo sapiens (human) ]
• Official Symbol: WARS2
• Synonyms: WARS2; tryptophanyl tRNA synthetase 2, mitochondrial; TrpRS; tryptophan--tRNA ligase, mitochondrial; (Mt)TrpRS; tryptophan tRNA ligase 2, mitochondrial
• Gene ID: 10352
• mRNA Refseq: NM_015836.3
• Protein Refseq: NP_056651.1
• UniProt ID: Q9UGM6