Recombinant Human VSIG4 Protein (Met1-Pro283), HlgG1 Fc-tagged, Biotinylated

• Cat.No.: VSIG4-1161H
• Product Overview: Recombinant Human VSIG4 Protein (NP_009199.1) (Met1-Pro283) was produced by HEK293 Cells expression system. This protein was expressed with the Fc region of human IgG1 at the C-terminus. The purified protein was biotinylated in vitro.

Specification

• Description: VSIG4 (V-set and immunoglobulin domain containing 4), also known as complement receptor of the immunoglobulin superfamily (CRIg) and Z39Ig, is a type I transmembrane glycoprotein. It is a B7 family-related protein and an Ig superfamily member. In contrast to the B7 family members which contain two IgG domains, VSIG4 contains one complete V-type Ig domain and a truncated C-type Ig domain. VSIG4 is exclusively expressed on tissue resident macrophages and binds to multimers of C3b and iC3b that are covalently attached to particle surfaces. No VSIG4 expression appears to be present in T and B cells. VSIG4 functions as a negative regulator of T cell activation, and may be involved in the maintenance of peripheral T cell tolerance, and is also identified as a potent suppressor of established inflammation. Mouse VSIG4 is synthesized as a 28 amino acid precursor that contains a signal sequence, an V-type Ig domain (aa 36-115), one potential N-linked glycosylation site, and a single transmembrane domain. The V-type Ig domain of mouse VSIG4 shares 86% and 8% aa sequence identity with the V-type Ig domains of rat and human VSIG4, respectively.
• Source: HEK293
• Species: Human
• Tag: Fc
• Predicted N Terminal: Arg 20
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human VSIG4 consists of 505 amino acids and predicts a molecular mass of 56.2 kDa.
• Protein length: Met1-Pro283
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: VSIG4 V-set and immunoglobulin domain containing 4 [ Homo sapiens ]
• Official Symbol: VSIG4
• Synonyms: VSIG4; Z39IG; protein Z39Ig; CRIg
• Gene ID: 11326
• mRNA Refseq: NM_001100431
• Protein Refseq: NP_001093901
• MIM: 300353
• UniProt ID: Q9Y279